Congratulate you and your whole team on the grand success of the Conference held in Philadelphia, USA. It was well planned and organized. Since all members of the managing committee were very friendly and sincere we felt homely at all moments. Moreover, my especial thanks are due to you and your team for the kind cooperation, hospitality and management. I look forward and wish you the best for the next meeting to be held in 2016. Once again, I thank you and all the crew members associated with the 4th International Conference and Exhibition on Metabolomics & Systems Biology. With my sincere regards and best wishes, to you and your team.
Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  :10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity.